Translational_Unit

Part:BBa_K2848004:Design

Designed by: Lars Hoeing, Liv Paul, Luca Schelle   Group: iGEM18_Stuttgart   (2018-10-09)


Pre-NisA under control of Arabinose promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Restriction cloning in previous BioBricks containing an arabinose promotor and replacing the existing coding region.


Source

Lactococcus lactis

References